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Volume 46 Issue 11
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ZHANG Yu, ZHANG Qing, WANG Yijie, CAI Guojun, DONG Xiaoqiang, DU Yanjun, JIANG Ningjun. Engineering properties and environmental safety of biostimulated MICP-treated lead-contaminated soil[J]. Chinese Journal of Geotechnical Engineering, 2024, 46(11): 2352-2360. DOI: 10.11779/CJGE20230749
Citation: ZHANG Yu, ZHANG Qing, WANG Yijie, CAI Guojun, DONG Xiaoqiang, DU Yanjun, JIANG Ningjun. Engineering properties and environmental safety of biostimulated MICP-treated lead-contaminated soil[J]. Chinese Journal of Geotechnical Engineering, 2024, 46(11): 2352-2360. DOI: 10.11779/CJGE20230749
shu

Engineering properties and environmental safety of biostimulated MICP-treated lead-contaminated soil

  • 1.

    Institute of Geotechnical Engineering, Southeast University, Nanjing 211189, China

  • 2.

    Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hong Kong 999077, China

  • 3.

    School of Civil Engineering, Anhui Jianzhu University, Hefei 230601, China

  • 4.

    College of Civil Engineering, Taiyuan University of Technology, Taiyuan 030024, China

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  • Received Date: August 03, 2023
  • Available Online: January 09, 2024
    Graphical Abstract
    • Abstract
  • With the acceleration of urbanization, the remediation, development and reuse of heavy metal-contaminated sites have attracted increasing attention. Taking the typical heavy metal contaminant lead as the research object, the effects of lead concentration on the bacterial activity indexes such as pH of soil, number of viable cell, concentration of urea and ammonium are investigated by using the biostimulated MICP method. The unconfined compressive strength, permeability and toxicity leaching concentration of the solidified lead-contaminated soil are used to evaluate the solidification effects. The findings demonstrate that the biostimulation method can realize the enrichment of ureolytic bacteria in low lead concentration contaminated soils. The number of viable cell can reach 109 CFU/g after 7 days of enrichment, but the high concentration of lead contaminants significantly inhibits the growth and activity of microorganisms. The strength and impermeability show an increasing trend with solidifying time, and the strength of 40 mM lead-contaminated soil increases significantly and the permeability coefficient can be decreased to 6.5×10-6 m/s after solidifying for 14 days. The leaching concentration also decreases with solidifying time, and the leaching concentration of the low lead concentration-contaminated soil solidified for 14 days can be lower than 0.1 mg/L in neutral or weakly acidic environment. Based on the engineering properties and environmental safety tests, the solidification mechanism of the lead-contaminated soil treated by the biostimulated MICP is revealed by combining the scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and microbial 16 s whole genome resequencing.
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    • References (31)
  • [1]
    JIANG N J, LIU R, DU Y J, et al. Microbial induced carbonate precipitation for immobilizing Pb contaminants: toxic effects on bacterial activity and immobilization efficiency[J]. The Science of the Total Environment, 2019, 672: 722-731. doi: 10.1016/j.scitotenv.2019.03.294
    [2]
    WU R R, YAO F T, LI X Y, et al. Manganese pollution and its remediation: a review of biological removal and promising combination strategies[J]. Microorganisms, 2022, 10(12): 2411. doi: 10.3390/microorganisms10122411
    [3]
    ZHANG Y, HU X L, WANG Y J, et al. A critical review of biomineralization in environmental geotechnics: applications, trends, and perspectives[J]. Biogeotechnics, 2023, 1: 100003. doi: 10.1016/j.bgtech.2023.100003
    [4]
    JIANG N J, WANG Y J, CHU J, et al. Bio-mediated soil improvement: an introspection into processes, materials, characterization and applications[J]. Soil Use and Management, 2022, 38(1): 68-93. doi: 10.1111/sum.12736
    [5]
    刘汉龙, 肖鹏, 肖杨, 等. 微生物岩土技术及其应用研究新进展[J]. 土木与环境工程学报(中英文), 2019, 41(1): 1-14.

    LIU Hanlong, XIAO Peng, XIAO Yang, et al. State-of-the-art review of biogeotechnology and its engineering applications[J]. Journal of Civil and Environmental Engineering, 2019, 41(1): 1-14. (in Chinese)
    [6]
    李驰, 田蕾, 董彩环, 等. MICP技术联合多孔硅吸附材料对锌铅复合污染土固化/稳定化修复的试验研究[J]. 岩土力学, 2022, 43(2): 307-316.

    LI Chi, TIAN Lei, DONG Caihuan, et al. Experimental study on zinc-lead composite contaminated soil solidified/stabilized by MICP technology combined with porous silicon adsorption materials[J]. Rock and Soil Mechanics, 2022, 43(2): 307-316. (in Chinese)
    [7]
    KANG C, KWON Y J, SO J. Bioremediation of heavy metals by using bacterial mixtures[J]. Ecological Engineering, 2016, 89: 64-69. doi: 10.1016/j.ecoleng.2016.01.023
    [8]
    SHARMA M, SATYAM N, REDDY K R, et al. Multiple heavy metal immobilization and strength improvement of contaminated soil using bio-mediated calcite precipitation technique[J]. Environmental Science and Pollution Research International, 2022, 29(34): 51827-51846. doi: 10.1007/s11356-022-19551-x
    [9]
    CHEN X, ACHAL V. Effect of simulated acid rain on the stability of calcium carbonate immobilized by microbial carbonate precipitation[J]. Journal of Environmental Management, 2020, 264: 110419. doi: 10.1016/j.jenvman.2020.110419
    [10]
    土工试验方法标准: GB/T 50123—2019[S]. 北京: 中国计划出版社, 2019.

    Standard for Geotechnical Testing Method: GB/T 50123—2019[S]. Beijing: China Planning Press, 2019. (in Chinese)
    [11]
    陈敏洁, 李亚飞, 李博文, 等. 微生物诱导碳酸钙沉淀对土壤中Pb污染稳定化的效果研究[J]. 有色金属工程, 2020, 10(12): 128-134. doi: 10.3969/j.issn.2095-1744.2020.12.018

    CHEN Minjie, LI Yafei, LI Bowen, et al. Effect of stabilization of Pb-pollution in soil based on microbial induced calcite precipitation[J]. Nonferrous Metals Engineering, 2020, 10(12): 128-134. (in Chinese) doi: 10.3969/j.issn.2095-1744.2020.12.018
    [12]
    YANG Z P, WANG Y, LI D H, et al. Influence of freeze-thaw cycles and binder dosage on the engineering properties of compound solidified/stabilized lead-contaminated soils[J]. International Journal of Environmental Research and Public Health, 2020, 17(3): 1077. doi: 10.3390/ijerph17031077
    [13]
    SADIGHI H, ROWSHANZAMIR M, BANITALEBI- DEHKORDI M. A multi-aspect application of microwave radiation on rehabilitating and improving the geotechnical properties of polluted-sand-clay mixture[J]. Journal of Contaminant Hydrology, 2022, 249: 104040. doi: 10.1016/j.jconhyd.2022.104040
    [14]
    陈蕾, 杜延军, 刘松玉, 等. 水泥固化铅污染土的基本应力-应变特性研究[J]. 岩土力学, 2011, 32(3): 715-721. doi: 10.3969/j.issn.1000-7598.2011.03.013

    CHEN Lei, DU Yanjun, LIU Songyu, et al. Experimental study of stress-strain properties of cement treated lead- contaminated soils[J]. Rock and Soil Mechanics, 2011, 32(3): 715-721. (in Chinese) doi: 10.3969/j.issn.1000-7598.2011.03.013
    [15]
    WANG Y J, JIANG N J, HAN X L, et al. Shear behavior of bio-cemented calcareous sand treated through bio-stimulation under the direct shear condition[J]. Bulletin of Engineering Geology and the Environment, 2022, 81(10): 413. doi: 10.1007/s10064-022-02907-5
    [16]
    WANG Y J, JIANG N J, SARACHO A C, et al. Compressibility characteristics of bio-cemented calcareous sand treated through the bio-stimulation approach[J]. Journal of Rock Mechanics and Geotechnical Engineering, 2023, 15(2): 510-522. doi: 10.1016/j.jrmge.2022.05.007
    [17]
    WANG Y J, HAN X L, JIANG N J, et al. The effect of enrichment media on the stimulation of native ureolytic bacteria in calcareous sand[J]. International Journal of Environmental Science and Technology, 2020, 17(3): 1795-1808. doi: 10.1007/s13762-019-02541-x
    [18]
    FANG L Y, NIU Q J, CHENG L, et al. Ca-mediated alleviation of Cd2+ induced toxicity and improved Cd2+ biomineralization by Sporosarcina pasteurii[J]. Science of the Total Environment, 2021, 787: 147627. doi: 10.1016/j.scitotenv.2021.147627
    [19]
    American Society for Testing and Materials (ASTM). ASTM D4972-19 Standard test methods for pH of soils [S]. West Conshohocken: ASTM, 2019.
    [20]
    李元芳. 有效活菌数的测定方法、允许差与判定[J]. 土壤肥料, 1997(4): 43-44.

    LI Yuanfang. Determination method, allowable difference and judgment of effective viable bacteria number[J]. Soil and Fertilizer Sciences in China, 1997(4): 43-44. (in Chinese)
    [21]
    American Society for Testing and Materials (ASTM). ASTM D5084-16a Standard test methods for measurement of hydraulic conductivity of saturated porous materials using a flexible wall permeameter[S]. West Conshohocken: ASTM, 2016.
    [22]
    固体废物浸出毒性浸出方法水平振荡法: HJ 557—2010[S]. 北京: 中国环境科学出版社, 2010.

    Solid Waste-Extraction Procedure for Leaching Toxicity-Horizontal Vibration Method: HJ 557—2010[S]. Beijing: China Environmental Science Press, 2010. (in Chinese)
    [23]
    固体废物浸出毒性浸出方法醋酸缓冲溶液法: HJ/T 300—2007[S]. 北京: 中国环境科学出版社, 2007.

    Solid Waste-Extraction Procedure for Leaching Toxicity-Acetic Acid Buffer Solution Method: HJ/T 300—2007[S]. Beijing: China Environmental Science Press, 2007. (in Chinese)
    [24]
    固体废物浸出毒性浸出方法醋酸缓冲溶液法: HJ/T 300—2007[S]. 北京: 中国环境科学出版社, 2007.

    Solid Waste-Extraction Procedure for Leaching Toxicity-Acetic Acid Buffer Solution Method: HJ/T 300—2007[S]. Beijing: China Environmental Science Press, 2007. (in Chinese)
    [25]
    TANG C S, YIN L Y, JIANG N J, et al. Factors affecting the performance of microbial-induced carbonate precipitation (MICP) treated soil: a review[J]. Environmental Earth Sciences, 2020, 79(5): 94. doi: 10.1007/s12665-020-8840-9
    [26]
    KANG C H, OH S J, SHIN Y, et al. Bioremediation of lead by ureolytic bacteria isolated from soil at abandoned metal mines in South Korea[J]. Ecological Engineering, 2015, 74: 402-407. doi: 10.1016/j.ecoleng.2014.10.009
    [27]
    地下水质量标准: GB/T 14848—2017[S]. 北京: 中国标准出版社, 2017.

    Standard for Groundwater Quality: GB/T 14848— 2017[S]. Beijing: Standards Press of China, 2017. (in Chinese)
    [28]
    YUAN K, LEE S S, DE ANDRADE V, et al. Replacement of calcite (CaCO3) by cerussite (PbCO3)[J]. Environmental Science & Technology, 2016, 50(23): 12984-12991.
    [29]
    LI X X, WANG Y, TANG J J, et al. Removal behavior of heavy metals from aqueous solutions via microbially induced carbonate precipitation driven by acclimatized sporosarcina pasteurii[J]. Applied Sciences, 2022, 12(19): 9958. doi: 10.3390/app12199958
    [30]
    XIAO Y, HE X, ZAMAN M, et al. Review of strength improvements of biocemented soils[J]. International Journal of Geomechanics, 2022, 22(11): 03122001. doi: 10.1061/(ASCE)GM.1943-5622.0002565
    [31]
    董博文, 刘士雨, 俞缙, 等. 靶向激活产脲酶微生物加固钙质砂试验研究[J]. 岩土工程学报, 2021, 43(7): 1315-1321. doi: 10.11779/CJGE202107017

    DONG Bowen, LIU Shiyu, YU Jin, et al. Experimental study on reinforcement of calcareous sand by targeting activation of microbes producing urease[J]. Chinese Journal of Geotechnical Engineering, 2021, 43(7): 1315-1321. (in Chinese) doi: 10.11779/CJGE202107017
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