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张瑾璇, 刘汉龙, 肖杨. 液滴微流控芯片系统研发与微生物矿化机理研究[J]. 岩土工程学报, 2024, 46(6): 1236-1245. DOI: 10.11779/CJGE20230255
引用本文: 张瑾璇, 刘汉龙, 肖杨. 液滴微流控芯片系统研发与微生物矿化机理研究[J]. 岩土工程学报, 2024, 46(6): 1236-1245. DOI: 10.11779/CJGE20230255
ZHANG Jinxuan, LIU Hanlong, XIAO Yang. Development of droplet microfluidic system and regime of biomineralization[J]. Chinese Journal of Geotechnical Engineering, 2024, 46(6): 1236-1245. DOI: 10.11779/CJGE20230255
Citation: ZHANG Jinxuan, LIU Hanlong, XIAO Yang. Development of droplet microfluidic system and regime of biomineralization[J]. Chinese Journal of Geotechnical Engineering, 2024, 46(6): 1236-1245. DOI: 10.11779/CJGE20230255

液滴微流控芯片系统研发与微生物矿化机理研究

Development of droplet microfluidic system and regime of biomineralization

  • 摘要: 微生物诱导碳酸钙沉积(MICP)技术是一种基于生物矿化作用的新型土体加固和修复技术。由于生物反应过程复杂,结晶影响因素多,MICP矿化机理目前还不明确。MICP微流控系统能够对微细尺度下的微生物矿化沉积规律进行可视化研究,但观测晶体生长尺度仍较大。设计了一种液滴微流控芯片,通过调控不相溶两相流体的流速,生成了直径约450 μm的扁球体弹状流液滴作为MICP微反应器,从而搭建了精度更高的微生物矿化液滴微流控芯片系统。通过该系统观测表明:碳酸钙结晶点周围的细菌分布均无变化,随着反应进行,晶体尺寸逐渐扩大,形貌不变;在晶体生长过程中,部分细菌吸附到晶体表面,但晶体周围无明显的细菌聚集现象。此外,采用扫描电镜进一步观测到碳酸钙表面细菌吸附部位存在孔洞。液滴微流控芯片系统为微生物矿化提供了一个精细的微米尺度反应器,为探究MICP成核机理提供了一种有效的分析方法。

     

    Abstract: The microbially induced calcium carbonate precipitation (MICP) is a novel technique for soil reinforcement based on biomineralization. The MICP regime has not been fully explored due to the complexity of biomineralization process with so many factors affecting nucleation and growth of minerals. Recently, biomineralization evolution can be visually observed at microscale by using the microfluidic system, whereas the magnification for observation of crystal growth is still limited. In this study, a droplet microfluidic chip is designed to generate oblate droplet cells with 450μm in diameter by adjusting the flow velocities of two immiscible liquids, which can be used for MICP microreactor, i.e., the droplet microfluidic system for biomineralization with high precision. With the help of the system, it is found that the distribution of bacteria around CaCO3 crystals is almost unchanged with the CaCO3 crystals growing proportionally, and the crystal morphology remains the same. Some bacterial cells nearby the crystal are adsorbed on the crystal surface during the crystal growth with no obvious bacterial aggregation around the crystal. In addition, with the help of SEM, some caves on the crystal surface are found to be the sites that bacterial cells are adsorbed. Consequently, the droplet microfluidic system can provide a precise microreactor for biomineralization and an effective method for exploring nucleation regimes in MICP.

     

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