Abstract: To study the earthquake damage response of a subway station structure in a liquefied interlayer site, a series of dynamic centrifuge tests are carried out using the ZJU400 geotechnical centrifuge facility in Zhejiang University. The test scheme and the process of carrying out the tests are introduced. At the same time, in view of the current situation that there is no precedent for a reference for the preparation of the liquefied interlayer site, many technical details are elaborated, including the preparation of liquefied interlayer site (clay consolidation, sand saturation and assembly of interlayer site), structural waterproof treatment, and releasing initial pore pressure in the sand layer during centrifugal loading. The tests obtain ideal results, thus achieving the experimental goal of studying the earthquake damage response of the subway station structure in the liquefied interlayer site. The introduction to the design process of this test scheme may provide some references for the similar tests in the future.